3. Example of a Complete Peptide Sequence Analysis Using Complementary Application of ISD and the PPSQ Protein Sequencer
- 1. What Is a Protein Sequencer?
- 2. Differences from Mass Spectrometry-Based Amino Acid Sequence Analysis
- 3. Example of a Complete Peptide Sequence Analysis Using Complementary Application of ISD and the PPSQ Protein Sequencer
This example shows how the complete sequence of a peptide was determined using a combination of the PPSQ and MALDI-TOF MS. By analyzing the sample on both instruments and combining the results in a complementary manner, complete sequence information can be obtained with greater confidence.
Structure of Cyclic Peptide BNP
Sequence Analysis of BNP Using a Gradient System
Cycle 1: Raw chromatogram; Cycle 2 and subsequent cycles: Subtracted chromatograms
ISD Spectrum of BNP Obtained by MALDI-TOF MS
Sequence Determination of BNP by Combined ISD and Edman Degradation Sequencing