LC/MS/MS Method Package for D/L Amino Acids
For LabSolutions™ LCMS Software
Most important amino acids exist as stereoisomers. D- and L- forms of mirror image isomers, or enantiomers, are named according to their activity on polarized light. By using CROWNPAK CR-I(+) and CR-I(-) columns with chiral stationary phases, the D- and L-forms of amino acids can be analyzed separately. With CR-I(+) elution order is from D- to L-, and with CR-I(-) the elution order is reversed.
In Just Ten Minutes, Chiral Amino Acids Can Be Analyzed Simultaneously
With conventional chiral amino acid analysis, it is necessary to perform derivatization or use very long run times. With this method package, derivatization is not necessary, and high-sensitivity analysis can be performed in a short period of time, bringing efficiency to the chiral separations laboratory.
All D/L Amino Acids Can Be Quantified by Column Switching
The physicochemical properties of Glutamine and Lysine, Isoleucine and allo-Isoleucine, Threonine and allo-Threonine are extremely similar. They have virtually the same MS/MS fragmentation patterns, and share many of their MRM transitions. Chromatographic separation is therefore required to analyze them individually by LC-MS/MS. Even if there is coelution on the CR-I(+) column, confirmation can be made by automated switching to a secondary CR-I(-) column.
Analysis of D/L-Isoleucine, D/L-allo-Isoleucine, and D/L-Leucine |
|
Analysis of D/L-Threonine, and D/L-allo-Threonine |
List of Registered Amino Acids
- D/L-Alanine
- D/L-Arginine
- D/L-Asparagine
- D/L-Aspartic acid
- D/L-Cysteine
- D/L-Glutamine
- D/L-Glutamic acid Glycine
- Glycine
- D/L-Histidine
- D/L-Isoleucine
- D/L-allo-Isoleucine
- D/L-Leucine
- D/L-Lysine
- D/L-Methionine
- D/L-Phenylalanine
- DL-Proline
- D/L-Serine
- D/L-Threonine
- D/L-allo-Threonine
- D/L-Tryptophane
- D/L-Tyrosine
- D/L-Valine
Precautions
1. DL-Proline is a secondary amine, so it cannot be separated with these analysis conditions.
2. LabSolutions LCMS Ver. 5.86 or later is required.
The analysis method of this method package was developed based on the reserach of the Fukusaki Lab, Division of Science and Biotechnology, Graduate School of Engineering, Osaka University.
Reference: Nakano,Y., Konya,Y., Taniguchi,M., Fukusaki,E., Journal of Bioscience and Bioengineering , 123, 134-138 (2016)
LabSolutions and LCMS are trademarks of Shimadzu Corporation.
CROWNPAK is a registered trademark of Daicel Corporation.
News / Events
-
Three Analytical and Measuring Instruments Win the Internationally Recognized “iF Design Award 2024”
The Shimadzu LCMS-2050 high-performance liquid chromatograph mass spectrometer, the Brevis GC-2050 gas chromatograph, and the ICPMS-2040/2050 ICP mass spectrometer won the internationally recognized iF Design Award 2024 in the product design category.
-
GCMS-QP2050 Gas Chromatograph Mass Spectrometer Video has released
The business environments and needs involved in analysis work change on a continual basis. The next-generation GCMS-QP2050 gas chromatograph mass spectrometer, with its accumulation of impressive Shimadzu technology, will lead the way forward. New value is provided by hardware boasting astounding reliability and stability, and easy-to-operate software equipped with superior automated technology.
-
OAD-TOF system Interview with Dr.Hiroshi Tsugawa
The OAD-TOF system is a Q-TOF LCMS that realizes OAD (Oxygen Attachment Dissociation), Shimadzu's proprietary fragmentation technology. It allows the analysis of the position of carbon-carbon double bonds in lipids and other organic compounds. Combined with the LCMS -9050, which boasts world-class high mass accuracy and fast polarity switching, it enables the creation of innovative applications.
-
Metabolic Pathway Analysis Solutions
Metabolic pathways are the chain of chemical and enzymatic reactions that occur within a cell in living organisms to support their life.
-
AOAC INTERNATIONAL Posters are now available.
-
Shimadzu has released the OAD-TOF system
The OAD-TOF system is a Q-TOF LCMS that realizes OAD (Oxygen Attachment Dissociation), Shimadzu's proprietary fragmentation technology. It allows the analysis of the position of carbon-carbon double bonds in lipids and other organic compounds. Combined with the LCMS-9050, which boasts world-class high mass accuracy and fast polarity switching, it enables the creation of innovative applications.