Nexera X4 Ultra High-Performance Liquid Chromatograph - Features
Ultra High-Performance Liquid Chromatograph
Ultra-Sharp Peaks
Reduces peak dispersion to achieve excellent chromatographic separation
Improves Separation by Minimizing Peak Broadening
The Nexera X4 is equipped with Nexflow technology, which achieves low dispersion without changing the internal diameter of the flow lines, thereby minimizing system-derived peak broadening. This allows the performance of UHPLC columns to be fully utilized, resulting in high separation performance.
Chromatogram of Impurities in a Pharmaceutical Sample
Maximizes Column Performance
Columns play a critical role in achieving high-speed, high-resolution analysis by minimizing band broadening within the column and delivering superior chromatographic separation. The Nexera X4 enhances column performance by minimizing broadening of sample peaks outside the column. In particular, it improves theoretical plate numbers for weakly retained components in isocratic analysis, where extra-column band broadening tends to be the dominant factor.
Ultra-Fast Analysis
Higher productivity with consistent ultra-fast gradients
Dramatically Improves Productivity
Four independently actuated plungers and a pressure feedback mechanism significantly improve solvent delivery responsiveness and enable ultra-fast analysis. Analysis times are 92 % and 78 % shorter compared to those achieved with typical HPLC and existing UHPLC-like systems, respectively, resulting in up to 14 times higher laboratory productivity.
Achieves Consistent Analytical Results Even for Ultra-Fast Gradients
The Nexera X4 enables gradient analysis with highly consistent solvent delivery, resulting in excellent retention time reproducibility, even for ultra-fast analysis. The combination of significantly faster speeds and analysis quality equivalent to conventional HPLC dramatically improves productivity.
Ultra-Low Solvent Consumption
In combination with narrow-bore columns, it significantly reduces the quantity of solvent consumed
Provides the Instrument Performance Required for Analytical Miniaturization
Analytical Miniaturization requires precise injection of micro quantities compatible with narrow-bore columns, accurate delivery of mobile phases, and prevention of band broadening within flow channels. The Nexera X4 offers exceptional instrument performance that satisfies all of those requirements, even when using narrow-bore columns.
Micro-injection
Achieve micro-injection for analytical miniaturization with a high-precision measuring pump. Good repeatability is obtained with injection amounts of 1 μL or less.
Precise Solvent Delivery
Precise solvent delivery is possible at minute flow rates. The small internal volume makes the system suitable for gradient analysis with narrow-bore columns.
Reduced Peak Broadening
Nexflow technology reduces peak broadening. Separation performance remains excellent even after analytical miniaturization.
Reducing Solvent Consumption with Narrow-Bore Columns
Shimadzu provides narrow-bore columns with a 1-mm internal diameter as part of the Shim-pack Scepter™ series of columns, packed with a fully porous hybrid organosilica base material, and the Shim-pack™ NovaCore series of core-shell columns, also packed with a hybrid organosilica base material. Used in combination with the Nexera X4, these columns enable analytical miniaturization and significantly reduce solvent consumption.
Best Partner for Mass Spectrometry
Combinations that Maximize Mass Spectrometer Performance
Nexflow Tubing for MS
The Nexflow Tubing for MS is a dedicated tubing kit for Nexera X4 systems that reduces peak broadening between the column outlet and MS interface. By fully leveraging the ultra-fast and high-separation performance of the Nexera X4, the kit maximizes mass spectrometer performance.
Combining Nexera X4 and LCMS-8060 Series Systems for High-Speed and High-Sensitivity Analysis
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The triple quad LCMS-8060 series achieves positive and negative ionization switching in just 5 msec with UFswiching™ technology. Suppressing ion intensity loss, even during ultra-fast switching, enables users to consistently obtain stable, high-sensitivity data. The combination of an LCMS-8060 system with the Nexera X4, which achieves excellent gradient control performance, results in a next-generation LCMS platform that delivers unmatched throughput and reliability.
Improved Efficiency of Pharmacokinetic Analysis
Pharmacokinetic research demands fast, reliable analysis to keep pace with large sample volumes. Using the Nexera X4 in combination with the LCMS-8060RX, eight anticoagulants were analyzed with outstanding accuracy and precision, even with straightforward protein precipitation sample preparation. With a total analysis time of just 2.45 minutes, including column equilibration, this solution enables true high-throughput pharmacokinetic workflows.
