Nexera X4 Ultra High-Performance Liquid Chromatograph - Specs

Ultra High-Performance Liquid Chromatograph

Specifications

Engineering Excellence

Innovative design pursuing maximum performance

Exhibiting Maximum Performance while Maintaining Instrument Robustness

Nexera X4 systems incorporate proprietary Nexflow technology that minimizes extra-column band broadening without changing the tube’s internal diameter or length. By reducing the risk of blockages and minimizing peak broadening, the Nexera X4 delivers outstanding separation performance.

Solvent Delivery Unit Achieves High-Speed High-Precision Gradients

The LC-40B X4 is a binary gradient pump featuring four independently actuated plungers and a pressure feedback mechanism. Independent plunger actuation greatly reduces pump-derived pulsation and improves gradient responsiveness. These advanced fluid control technologies deliver stable baselines for ultra-fast gradients and consistent, reliable performance under high-pressure conditions.

Mobile Phase Switching and Degassing Unit Broadens the Analysis Width

The SSU-40 is a solvent switching and online degassing unit for binary pumps that can switch between up to 4 solutions per pump. Automatic mobile phase switching helps improve the efficiency of method screening.

Newly Designed Low-Dispersion,High-Sensitivity PDA Detector

The SPD-M40 X4 is a photodiode array detector that uses a capillary cell to reduce peak broadening. This design minimizes solvent-derived refractive index effects, leading to stable baselines and higher sensitivity. In addition, the broader dynamic range enables analysis of a wider range of low to high-concentration samples.

High-End Autosampler Reduces Peak Broadening and Carryover

The SIL-40C X4 is a high-end autosampler featuring a newly designed low-internal-volume high-pressure valve, low-dispersion injection mode, and high-speed needle internal/external rinsing. These capabilities make the SIL-40C X4 suitable for a wide range of samples, from those with extremely low concentrations that require high sensitivity to samples with high concentrations that require rinsing functionality.
Easy-to-Use Fitting Achieves Low-Dispersion Performance

The newly developed "Nexlock™ II" employs an end-surface sealing structure that eliminates ferrules and minimizes dead volume in tubing connections. Its proprietary fitting design also allows tool-free column connections while maintaining high-pressure tolerance.

Autosampler
Plate Changer
Column Oven
PDA Detector

System Controller

	SCL-40	CBM-40	CBM-40lite
Monitor	Touch panel LabSolutions™ Web monitor	LabSolutions Web monitor	LabSolutions Web monitor
Controllable unit	Solvent delivery unit: Max. 4, Autosampler: 1, Column oven: Max. 4, Detector: Max. 2, etc.
Number of controllable units	8 (Using option: 12)		4 (excluding built-in solvent delivery unit)
Event input/output	Input: 1, Output: 2
Analog signal input	Up to two channels (option)	One channel (option)	ー
Communication interface	Ethernet
Reservoir tray	Included as standard
Dimensions and weight	W260 × D500 × H140 mm, 6 kg	W260 × D500 × H72 mm, 5 kg	ー
Operating temperature range	4 to 35 °C
Power supply	100 to 240 V AC, 50 VA, 50/60 Hz		Supplied from solvent delivery unit

Solvent Delivery Unit

	LC-40B X4
	Pumping method	Micro-volume double plunger pump
	Allowable maximum pressure	130 MPa
	Flow rate settings range	0.0001 to 2.0000 mL/min
	Flow rate accuracy	±1 % (at 1 mL/min, 80 MPa, with water, stable within a temperature range of 20 to 30 °C)
	Flow rate precision	Retention time reproducibility: ≤ 0.04 % RSD or ≤ 0.003 min SD, whichever is greater
	Gradient mode	Two-solvent high-pressure gradient
	Range of gradient mixing ratio	0 to 100 % (0.1 % step)
	Gradient concentration precision	≤ 0.1% RSD or ≤ 0.008 min SD, whichever is greater (under specified conditions)
	Wetted materials	SUS316L, Co-Ni alloy, PEEK, ruby, sapphire, zirconia, Hastelloy C, polyethylene, fluoropolymer
	Available pH range	1 to 14
	Automatic rinsing kit	Included as standard
	Degassing unit	1 unit connectable
	Dimensions and weight	W260 × D500 × H210 mm (excluding protrusions), 24 kg
	Operating temperature range	4 to 35 °C
	Power supply	100 to 240 V AC, 50/60 Hz, 150 VA

Solvent Switching and Degassing Unit

	SSU-40
	Number of selectable solvents	Up to 4 solvents per pump (maximum 2 pumps)
	Number of degassed solvent lines	5
	Wetted materials	Fluoropolymer, PEEK, PTFE, FEP, ETFE, perfluoroelastomer
	Degassed flow line capacity	400 μL/1 line
	Dimensions and weight	W260 × D500 × H72 mm, 5 kg
	Operating temperature range	4 to 35 °C
	Power supply	Supplied from the corresponding solvent delivery unit

Autosampler

	SIL-40C X4
	Injection method	Total-volume injection
	Allowable maximum　pressure	130 MPa
	Injection volume settings range	0.01 to 50 μL
	Injection volume accuracy	Within ±1% (5 μL injection, n = 20)
	Linearity	≥ 0.9999
	Injection cycle time	≤ 6.7 seconds (under specified conditions)
	Number of samples processed	288 (microtiter plate, 96 well × 3 plates) 1152 (microtiter plate, 384 well × 3 plates) 252 (1 mL sample vial, 84 × 3 plates) 162 (1.5 mL sample vial, 54 × 3 plates) 84 (4 mL sample vial, 28 × 3 plates) 36 (10 mL sample vial, 12 × 3 plates) 72 (1.5 mL micro tube, 24 × 3 plates)
	Injection volume reproducibility	RSD ≤ 1.0% (0.5 to 0.9 μL) RSD ≤ 0.5% (1.0 to 1.9 μL) RSD ≤ 0.25% (2.0 to 4.9 μL) RSD ≤ 0.15% (more than 5.0 μL)
	Cross-contamination	≤ 0.0010 % (without rinse) ≤ 0.0003 % (with rinse, typically) (under specified conditions)
	Dip rinsing outside the needle and injection port rinsing	Included as standard
	External needle flushing	Included as standard
	Internal needle flushing (3 solvents)	Included as standard
	Sample cooler	Included as standard (air-circulation temperature control type)
	Sample cooler temperature setting range	4 to 45 °C (To set 4 °C, room temperature needs to be below 30 °C and humidity below 70 %)
	Sample cooler temperature accuracy	±2 °C (sensor position ±0.5 °C)
	Wetted materials	Mobile phase flow line: SUS316L, DLC, PEEK Other flow lines: SUS316L, PTFE, ETFE, FEP, GFP, sapphire, PPS, FEKM
	Available pH range	1 to 14
	Dimensions and weight	W260 × D500 × H280 mm (protrusion adds 140 mm to the depth), 24 kg
	Operating temperature range	4 to 35 °C
	Power supply	100 to 240 V AC, 50/60 Hz, 400 VA

Plate Changer

PLATE CHANGER
Number of samples processed (including 2 plates of autosampler)	Using 1 plate changer: 1536 (microtiter plate, 96 well × 16 plates) 864 (deep-well plate, 96 well × 9 plates) 6144 (microtiter plate, 384 well × 16 plates) 3456 (deep-well plate, 384 well × 9 plates) 756 (1 mL sample vial, 84 × 9 plates) 486 (1.5 mL sample vial, 54 × 9 plates) 252 (4 mL sample vial, 28 × 9 plates) 108 (10 mL sample vial, 12 × 9 plates)	Using 3 plate changer: 4224 (microtiter plate, 96 well × 44 plates) 2208 (deep-well plate, 96 well × 23 plates) 16896 (microtiter plate, 384 well × 44 plates) 8832 (deep-well plate, 384 well × 23 plates) 1932 (1 mL sample vial, 84 × 23 plates) 1242 (1.5 mL sample vial, 54 × 23 plates) 644 (4 mL sample vial, 28 × 23 plates) 276 (10 mL sample vial, 12 × 23 plates)
Sample cooler	Air-circulation temperature control type, 4 to 45 °C (To set 4 °C, room temperature needs to be below 30 °C and humidity below 70 %)
Dimensions and weight	W170 × D500 × H560 mm (protrusion adds 140 mm to the depth), 26 kg
Operating temperature range	4 to 35 °C
Dimensions and weight	W260 × D500 × H72 mm, 5 kg
Operating temperature range	4 to 35 °C
Power supply (for 1 unit)	100 to 240 V AC, 400 VA, 50/60 Hz

Column Oven

	CTO-40C	CTO-40S
Temperature control type	Forced air circulation
Cooling method	Electronic cooling
Temperature control range	Room temperature—10 to 100 °C	Room temperature—10 to 85 °C
Temperature accuracy	±0.5 °C	±0.8 °C
Temperature precision	0.1 °C (±0.05 °C)	0.2 °C (±0.1 °C)
Containable column size and number	6 columns, each up to 250 mm long, or 3 columns, each up to 300 mm long	6 columns, each up to 100 mm long, or 3 columns, each up to 300 mm long
Dimensions and weight	W260 × D500 × H415 mm, 21 kg	W130 × D500 × H553 mm, 15 kg
Operating temperature range	4 to 35°C
Power supply	100 to 120 V AC/220 to 240 V AC (automatic switching) 400 VA, 50/60 Hz	100 to 240 V AC, 300 VA, 50/60 Hz

PDA Detector

	SPD-M40 X4
	Light source	D2 lamp
	Number of photodiode array elements	1024
	Wavelength range	190 to 800 nm
	Wavelength accuracy	≤ ±1 nm
	Wavelength reproducibility	≤ ±0.1 nm
	Slit width	1.2 nm, 8 nm
	Spectral resolution	≤ 1.4 nm
	Drift	≤ 0.5 × 10-3 of AU/h (under specified conditions)
	Noise	≤ 4.0 × 10-6 AU (under specified conditions)
	Linearity	3.0 AU (under specified conditions, typically)
	Flow cell	Optical path length: 10 mm, Cell volume: 0.5 μL, Maximum pressure: 8 MPa Material of wetted parts: PEEK, PCTFE, PFA, quartz
	Sampling rate	Max. 250 Hz
	Optional flow cell	High-sensitivity cell (Optical path length: 85 mm, Cell volume: 4.2 μL, Maximum pressure: 8 MPa)
	Available pH range	1 to 13 (Depending on the type of mobile phase, if used for a long period at pH 10 or higher, the quartz of the flow cell may be damaged, causing changes in transmission properties.)
	Dimensions and weight	W260 × D500 × H140 mm, 10 kg
	Operating temperature range	4 to 35 °C
	Power supply	100 to 240 V AC, 180 VA, 50/60 Hz

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