LC/MS/MS Method Package for Cell Culture Profiling Ver. 3

The validity of the analysis performed by each laboratory needs to be confirmed by Quality Control (QC). This article section shows the general analytical procedure for fulfilling QC requirements. Note that the procedure could be modified depending on the situation in the lab and analysts.

The CLAM-2040 accompanies you on most of the analytical workflow and improves your overall throughput by drastically reducing the sample preparation time. Simply place the blood tubes in position and the CLAM-2040 performs the next steps automatically, from sample extraction, up to the LCMS analysis and data processing.

Per-and polyfluoroalkyl substances (PFAS) are a group of persistent and harmful chemicals that can be commonly found in the environment globally. These anthropogenic chemicals can come from various sources such as manufacturing, industrial applications, food contact materials and consumer products, and eventually end up and accumulate in the water, soil and living organisms.

The LCMS-2050 mass spectrometer includes Mass-it functionality that can overlay compound mass (m/z) information, obtained with the mass spectrometer, onto UV chromatograms to support supplementing qualitative analysis results obtained using a UV detector.

The LCMS-2050 single quadrupole mass spectrometer is highly compact in size, yet provides both high-speed and high-sensitivity analysis. Shimadzu’s technology has been condensed into an instrument miniaturized to the utmost.

Version 3 of this method package adds metabolites characteristic of CHO cells, which are frequently used in the manufacture of monoclonal antibodies. Together with existing analytes, this Method Package enables the analysis of 144 compounds in under 20 minutes per sample.